Preparation of 35S-Methionine with Adsorption Chromatography on Activated Charcoal
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چکیده
منابع مشابه
The preparation of [35S]homocysteine thiolactone free of [35S]methionine.
The unambiguous study of homocysteine metabolism requires a source of [355]homocysteine completely free from contaminating [35S]methionine. Until now, such a reagent has not been readily available. Homocysteine is an important metabolite involved in methionine, cysteine and methylation metabolism. Homocysteine can be methylated to form methionine with either 5-methyltetrahydrofolate [1] or beta...
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I describe a convenient method for stripping hormones and other ligands from human serum. Formation of a serum/charcoal colloid is avoided by incubating serum on a limited surface area with activated charcoal, in a lateral oscillating shaker. Particle-free serum of negligible turbidity is obtained without ultracentrifugation by ejecting the centrifugate through a cellulose ester membrane. By th...
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The adsorption property of activated charcoal on verotoxin (VT)-producing Escherichia coli (VTEC) was examined using E. coli O157:H7. In the present study, E. coli O157:H7 strains were effectively adsorbed by activated charcoal. Adsorption was dose-dependent, and the maximum adsorption occurred within 5 min. At 10 mg of activated charcoal, bacteria tested were completely adsorbed. Activated cha...
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ژورنال
عنوان ژورنال: Journal of the agricultural chemical society of Japan
سال: 1967
ISSN: 0002-1407,1883-6844
DOI: 10.1271/nogeikagaku1924.41.8_406